Hotspot

trnS-GCU-trnT-CGU

LSC · 8982-9879 · 898 bp

Back to Senna

Pi 0.0826
MarkerSeek score 77.40
Primer available yes
Label trnS-GCU-trnT-CGU

Feature Metadata

label_name
trnS-GCU-trnT-CGU
feature_type
igs
region
LSC
length_bp
898
variable_sites
185
pi
0.0826
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.91
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-CGU_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 800-1027 bp 1.0 82.1
trnS-GCU-trnT-CGU_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 799-1026 bp 1.0 82.1
trnS-GCU-trnT-CGU_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 800-1027 bp 1.0 82.0
trnS-GCU-trnT-CGU_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 799-1026 bp 1.0 82.0
trnS-GCU-trnT-CGU_p5 CAATCCGACGCTTTAGTCCA AACGAATCGCACTTTTACCAC 50.0 42.9 58.0 57.5 801-1028 bp 1.0 79.7