Hotspot

trnQ-UUG-psbK

LSC · 7356-7970 · 615 bp

Back to Senna

Pi 0.0597
MarkerSeek score 79.20
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
615
variable_sites
110
pi
0.0597
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GATATGCTCTGGGACGGAAG AGCAGCTTGCCAAACAAAAG 55.0 45.0 57.6 58.1 847-1010 bp 1.0 88.0
trnQ-UUG-psbK_p2 GATATGCTCTGGGACGGAAG CTTACAGCAGCTTGCCAAAC 55.0 50.0 57.6 57.9 852-1015 bp 1.0 88.0
trnQ-UUG-psbK_p3 CCTCTAGTTTGGAGCCGGTA AGCAGCTTGCCAAACAAAAG 55.0 45.0 58.5 58.1 1358-1937 bp 1.0 87.4
trnQ-UUG-psbK_p4 CCTCTAGTTTGGAGCCGGTA CTTACAGCAGCTTGCCAAAC 55.0 50.0 58.5 57.9 1363-1942 bp 1.0 87.4
trnQ-UUG-psbK_p5 GATATGCTCTGGGACGGAAG TTCGATATTGGTGGGGGACT 55.0 50.0 57.6 58.1 1086-1253 bp 0.769231 78.3