Hotspot

trnK-UUU-rps16

LSC · 4323-5468 · 1146 bp

Back to Senna

Pi 0.0463
MarkerSeek score 72.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1146
variable_sites
165
pi
0.0463
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCTGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1368-1623 bp 1.0 91.5
trnK-UUU-rps16_p2 GGGCCCGATCCAATAACAAT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.0 58.1 1441-1696 bp 0.846154 85.4
trnK-UUU-rps16_p3 GGGCCCGATCCAATAACAAT TCCCAATGAGCCGTTTATCG 50.0 50.0 58.0 58.1 1442-1697 bp 0.846154 85.4
trnK-UUU-rps16_p4 CCTTTCTGGATCAGTCGTGG TGAGGTTGCGAATACGAGTG 55.0 50.0 58.0 58.0 1576-1831 bp 0.846154 85.0
trnK-UUU-rps16_p5 GGGCCCGATCCAATAACAAT TGAGGTTGCGAATACGAGTG 50.0 50.0 58.0 58.0 1649-1904 bp 0.692308 78.0