Hotspot

ndhF

SSC · 118435-120666 · 2232 bp

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Pi 0.0112

MarkerSeek score 50.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 37
pi: 0.0112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCTCGGGTTATGGAAGAAGG	TGGCGAATAAGAAACAAAACTCA	55.0	34.8	58.0	57.2	2938-3000 bp	0.666667	40.5
ndhF_p2	GGGACAGCTCATGATGTTCA	TGGCGAATAAGAAACAAAACTCA	50.0	34.8	57.6	57.2	2879-2941 bp	0.666667	37.7
ndhF_p3	TACGGGACAGCTCATGATGT	TGGCGAATAAGAAACAAAACTCA	50.0	34.8	58.5	57.2	2882-2944 bp	0.666667	36.9
ndhF_p4	TGTTTCGATGCAACAACCAA	ATTTATCCCCACTCTCTGGA	40.0	45.0	56.8	54.9	2941 bp	0.333333	18.5
ndhF_p5	TGTTTCGATGCAACAACCAA	TTTATCCCCACTCTCTGGAT	40.0	45.0	56.8	54.9	2940 bp	0.333333	18.5