Hotspot

ccsA-ndhD

SSC · 123364-123929 · 566 bp

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Pi 0.0561

MarkerSeek score 81.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 566
variable_sites: 46
pi: 0.0561
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AATGAGGCCTGGGGATCATA	CAGTTGACAAGGTCGAAGCT	50.0	50.0	57.9	58.1	835-854 bp	1.0	86.3
ccsA-ndhD_p2	TGAGGCCTGGGGATCATATT	CAGTTGACAAGGTCGAAGCT	50.0	50.0	57.9	58.1	833-852 bp	1.0	86.3
ccsA-ndhD_p3	AATGAGGCCTGGGGATCATA	TGGGCCCCGAGAGTTATTTA	50.0	50.0	57.9	58.1	921-940 bp	1.0	85.8
ccsA-ndhD_p4	TAATGAGGCCTGGGGATCAT	TGGGCCCCGAGAGTTATTTA	50.0	50.0	57.9	58.1	922-941 bp	1.0	85.8
ccsA-ndhD_p5	TGAGGCCTGGGGATCATATT	TGGGCCCCGAGAGTTATTTA	50.0	50.0	57.9	58.1	919-938 bp	1.0	85.8