Hotspot

ycf1-ndhF

SSC · 118324-118434 · 111 bp

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Pi 0.204
MarkerSeek score 72.70
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
111
variable_sites
27
pi
0.204
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 GCTCGGGTTATGGAAGAAGG TCCTATCCCCATGAATCGGA 55.0 50.0 58.0 57.3 1269-1323 bp 0.666667 67.1
ycf1-ndhF_p2 AGGGCCCTCTTATCTCTTCC TCCTATCCCCATGAATCGGA 55.0 50.0 57.9 57.3 1297-1351 bp 0.666667 66.5
ycf1-ndhF_p3 GGGCCCTCTTATCTCTTCCT TCCTATCCCCATGAATCGGA 55.0 50.0 57.9 57.3 1296-1350 bp 0.666667 66.5
ycf1-ndhF_p4 GCTCGGGTTATGGAAGAAGG AAAATATGTAGGGGGTGGCC 55.0 50.0 58.0 57.2 799 bp 0.333333 46.8
ycf1-ndhF_p5 GGGCCCTCTTATCTCTTCCT AAAATATGTAGGGGGTGGCC 55.0 50.0 57.9 57.2 826 bp 0.333333 46.3