Hotspot

accD-psaI

LSC · 65195-65750 · 556 bp

Back to Senegalia

Pi 0.0583

MarkerSeek score 80.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 556
variable_sites: 45
pi: 0.0583
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGCTTACATTGCATTTGCGG	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	786-869 bp	1.0	88.8
accD-psaI_p2	TGCTTACATTGCATTTGCGG	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	1078-1165 bp	1.0	87.9
accD-psaI_p3	TGCTTACATTGCATTTGCGG	ATACGTTCTGATCGCCAACC	45.0	50.0	58.0	58.1	1043-1130 bp	1.0	87.9
accD-psaI_p4	GTATGTGCTTCTGGAGGAGC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	980-1063 bp	1.0	87.5
accD-psaI_p5	TGCTTACATTGCATTTGCGG	CCAGGAAATCCCCAACGAAA	45.0	50.0	58.0	58.1	1352-1439 bp	1.0	86.8