Hotspot

trnS-GCU-trnG-UCC

LSC · 9659-10797 · 1139 bp

Back to Senegalia

Pi 0.0534
MarkerSeek score 72.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1139
variable_sites
51
pi
0.0534
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.74
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 700-1220 bp 1.0 80.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 699-1219 bp 1.0 80.0
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 689-1209 bp 1.0 78.3
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 688-1208 bp 1.0 78.3
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 689-1209 bp 1.0 78.3