Hotspot

ycf1

SSC · 107345-112411 · 5067 bp

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Pi 0.0108

MarkerSeek score 52.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5067
variable_sites: 229
pi: 0.0108
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACCGTCTCAAGGAAATCCC	TGCATATGGAATGTTCTCCCA	55.0	42.9	58.0	57.1	209-215 bp	1.0	69.9
ycf1_p2	GGACCGTCTCAAGGAAATCC	TGCATATGGAATGTTCTCCCA	55.0	42.9	58.0	57.1	210-216 bp	1.0	69.9
ycf1_p3	GGGACCGTCTCAAGGAAATC	TGCATATGGAATGTTCTCCCA	55.0	42.9	58.0	57.1	211-217 bp	1.0	69.9
ycf1_p4	TCGTCGAGCCGTAATCAAAG	TGCATATGGAATGTTCTCCCA	50.0	42.9	58.1	57.1	268-274 bp	1.0	68.4
ycf1_p5	CAGATTTTCGTCGAGCCGTA	TGCATATGGAATGTTCTCCCA	50.0	42.9	58.1	57.1	275-281 bp	1.0	68.3