Hotspot

rpl32-ndhF

SSC · 122892-123917 · 1026 bp

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Pi 0.0134

MarkerSeek score 56.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1026
variable_sites: 62
pi: 0.0134
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AAAAAGACTCCCCACCACAC	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.9	58.0	1511-1615 bp	0.916667	83.1
rpl32-ndhF_p2	AAAAAGACTCCCCACCACAC	CGCCCACACGATTAGTTACA	50.0	50.0	57.9	58.0	1969-2073 bp	0.916667	82.1
rpl32-ndhF_p3	TCTGCCCTTACCGAGAAAGA	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.1	58.0	1562-1674 bp	0.916667	82.1
rpl32-ndhF_p4	TCTGCCCTTACCGAGAAAGA	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.1	58.0	1560-1672 bp	0.916667	82.0
rpl32-ndhF_p5	TCTGCCCTTACCGAGAAAGA	CGCCCACACGATTAGTTACA	50.0	50.0	58.1	58.0	2020-2132 bp	0.916667	81.1