Hotspot

trnL-UAG-rpl32

SSC · 121889-122726 · 838 bp

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Pi 0.0186
MarkerSeek score 63.70
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
838
variable_sites
54
pi
0.0186
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC TCTACCGGGAATTCAAAAAGT 50.0 38.1 58.0 55.1 410-1004 bp 1.0 57.9
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA TCTACCGGGAATTCAAAAAGT 50.0 38.1 58.0 55.1 411-1005 bp 1.0 57.9
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA TCTACCGGGAATTCAAAAAGT 50.0 38.1 57.9 55.1 420-1014 bp 1.0 56.9
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC TCTACCGGGAATTCAAAAAGT 55.0 38.1 57.8 55.1 419-1013 bp 1.0 56.3
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC TCTACCGGGAATTCAAAAAGT 55.0 38.1 57.8 55.1 418-1012 bp 1.0 56.3