Hotspot

ndhD-ccsA

SSC · 120455-120727 · 273 bp

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Pi 0.0169

MarkerSeek score 66.90

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 273
variable_sites: 23
pi: 0.0169
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCTT	42.9	50.0	57.4	58.7	412-427 bp	1.0	69.5
ndhD-ccsA_p2	TCAGTTGACAAGGTTGAAGGT	CAATTGTGGCGACTCTAGGC	42.9	55.0	57.4	59.0	418-433 bp	1.0	67.4
ndhD-ccsA_p3	TCAGTTGACAAGGTTGAAGGT	GCAATTGTGGCGACTCTAGG	42.9	55.0	57.4	59.0	419-434 bp	1.0	67.4
ndhD-ccsA_p4	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCT	42.9	52.6	57.4	58.0	412-427 bp	1.0	67.2
ndhD-ccsA_p5	TCAGTTGACAAGGTTGAAGGT	GTGGCGACTCTAGGCTTTC	42.9	57.9	57.4	57.9	413-428 bp	1.0	66.9