Hotspot

ndhC-trnV-UAC

LSC · 50094-50843 · 750 bp

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Pi 0.0166
MarkerSeek score 66.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
750
variable_sites
52
pi
0.0166
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.9 57.8 803-845 bp 1.0 48.3
ndhC-trnV-UAC_p2 GACTTGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.2 57.8 835-877 bp 1.0 48.0
ndhC-trnV-UAC_p3 ACTTGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.2 57.8 834-876 bp 1.0 48.0
ndhC-trnV-UAC_p4 AGACTTGATATTATCAGAAATGCCC AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.0 57.8 836-878 bp 1.0 48.0
ndhC-trnV-UAC_p5 GACTTGATATTATCAGAAATGCCCA GAAGGTCTACGGTTCGAGTC 36.0 55.0 57.2 57.5 834-876 bp 1.0 48.0