Hotspot

psbZ-trnG-UCC

LSC · 35659-35951 · 293 bp

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Pi 0.0105

MarkerSeek score 40.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 293
variable_sites: 16
pi: 0.0105
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CAACCACTCAGCCATCTCTC	TATGGTAAAGGGGCGCATTC	55.0	50.0	58.0	58.0	1241-1265 bp	1.0	85.8
psbZ-trnG-UCC_p2	TCCATTTTTCTGGCTTGGCT	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	58.0	58.0	1357-1380 bp	1.0	85.5
psbZ-trnG-UCC_p3	TCCATTTTTCTGGCTTGGCT	TATGGTAAAGGGGCGCATTC	45.0	50.0	58.0	58.0	1421-1444 bp	1.0	85.3
psbZ-trnG-UCC_p4	CAACCACTCAGCCATCTCTC	TGACTTCATTTTTGGGCGGA	55.0	45.0	58.0	58.0	924-926 bp	1.0	84.1
psbZ-trnG-UCC_p5	TCCATTTTTCTGGCTTGGCT	TGACTTCATTTTTGGGCGGA	45.0	45.0	58.0	58.0	1104-1105 bp	1.0	83.7