Hotspot

trnT-GGU-psbD

LSC · 31181-32384 · 1204 bp

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Pi 0.0134

MarkerSeek score 60.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1204
variable_sites: 72
pi: 0.0134
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	TAACTCAGCGGTAGAGCACT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.2	58.0	1347-1389 bp	1.0	86.8
trnT-GGU-psbD_p2	TAACTCAGCGGTAGAGCACT	TGGACCAGAGAATGCGATTG	50.0	50.0	58.2	58.0	1713-1755 bp	1.0	85.1
trnT-GGU-psbD_p3	TAACTCAGCGGTAGAGCACT	AAACTAGGCGCAAAGAACCA	50.0	45.0	58.2	58.0	1784-1826 bp	1.0	84.9
trnT-GGU-psbD_p4	TAACTCAGCGGTAGAGCACT	AACTAGGCGCAAAGAACCAA	50.0	45.0	58.2	58.0	1783-1825 bp	1.0	84.9
trnT-GGU-psbD_p5	TAACTCAGCGGTAGAGCACT	ACCAAAACTAGGCGCAAAGA	50.0	45.0	58.2	58.0	1788-1830 bp	1.0	84.9