Hotspot

atpF-atpA

LSC · 28148-28224 · 77 bp

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Pi 0.0533

MarkerSeek score 71.40

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 77
variable_sites: 8
pi: 0.0533
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	ATATTGGCATATTGGGGGCG	TCGGCTTGAATGGTTACCA	50.0	47.4	58.1	56.7	133-141 bp	1.0	69.2
atpF-atpA_p2	ATTGGGGGCGATGAAACAAA	TCGGCTTGAATGGTTACCA	45.0	47.4	58.4	56.7	123-131 bp	1.0	67.3
atpF-atpA_p3	TTGGGGGCGATGAAACAAAT	TCGGCTTGAATGGTTACCA	45.0	47.4	58.4	56.7	122-130 bp	1.0	67.3
atpF-atpA_p4	TGGGGGCGATGAAACAAATA	TCGGCTTGAATGGTTACCA	45.0	47.4	57.5	56.7	121-129 bp	1.0	66.1
atpF-atpA_p5	TATTGGGGGCGATGAAACAA	TCGGCTTGAATGGTTACCA	45.0	47.4	57.5	56.7	124-132 bp	1.0	66.0