Hotspot

atpI-atpH

LSC · 25159-26257 · 1099 bp

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Pi 0.0113

MarkerSeek score 70.50

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1099
variable_sites: 57
pi: 0.0113
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTTGCAACTTTAGCCGCG	ATAACGGAAGCGGCAGAAAT	45.0	45.0	58.5	58.0	1177-1211 bp	1.0	82.5
atpI-atpH_p2	TTTTTGCAACTTTAGCCGCG	TACCTTGACCAACTCCAGGT	45.0	50.0	58.5	57.9	1233-1267 bp	1.0	81.6
atpI-atpH_p3	TTTTTGCAACTTTAGCCGCG	AGCCAATCCAGCAGCAATAA	45.0	45.0	58.5	57.9	1193-1227 bp	1.0	81.5
atpI-atpH_p4	TTTTTGCAACTTTAGCCGCG	AATAACGGAAGCGGCAGAAA	45.0	45.0	58.5	58.2	1178-1212 bp	1.0	81.3
atpI-atpH_p5	TCAGTGGTTCCTATCCCTGT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.4	58.0	1238-1272 bp	1.0	81.2