Hotspot

trnC-GCA-petN

LSC · 9335-10132 · 798 bp

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Pi 0.0227

MarkerSeek score 73.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 67
pi: 0.0227
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	836-874 bp	1.0	88.4
trnC-GCA-petN_p2	GTTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.0	58.2	893-931 bp	1.0	88.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	854-892 bp	1.0	86.0
trnC-GCA-petN_p4	TGTTGTGTATCGTTTTGGCG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.3	58.2	894-932 bp	1.0	83.2
trnC-GCA-petN_p5	GTTGTGTATCGTTTTGGCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.0	57.1	892-930 bp	1.0	82.4