Hotspot

rps16-trnQ-UUG

LSC · 6190-7162 · 973 bp

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Pi 0.0174

MarkerSeek score 62.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 55
pi: 0.0174
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1030-1046 bp	1.0	85.4
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1045-1061 bp	1.0	83.5
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1044-1060 bp	1.0	83.5
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1034-1050 bp	1.0	80.7
rps16-trnQ-UUG_p5	AGAATAAGAATCCACGGCGG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1062-1078 bp	0.833333	75.7