Hotspot

trnH-GUG-psbA

LSC · 77-330 · 254 bp

Back to Senecio

Pi 0.0225

MarkerSeek score 71.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 27
pi: 0.0225
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	373-543 bp	1.0	83.6
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	387-557 bp	1.0	83.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	386-556 bp	1.0	83.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	388-558 bp	1.0	83.3
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	55.0	50.0	58.1	57.6	374-544 bp	1.0	83.1