Hotspot

ycf1

SSC · 121235-126400 · 5166 bp

Back to Sempervivum

Pi 0.00998

MarkerSeek score 47.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5166
variable_sites: 125
pi: 0.00998
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTAGTTCACTCAATCCAACT	CCCCAAAACCAACCTTCCTT	38.1	50.0	53.9	58.2	206 bp	1.0	43.1
ycf1_p2	TCTAGTTCACTCAATCCAACT	TCACCCCAAAACCAACCTTC	38.1	50.0	53.9	58.2	209 bp	1.0	43.1
ycf1_p3	TCTAGTTCACTCAATCCAACT	TTCACCCCAAAACCAACCTT	38.1	45.0	53.9	57.7	210 bp	1.0	43.0
ycf1_p4	TCGTTTTTAGTTTCACTCCA	CCCCAAAACCAACCTTCCTT	35.0	50.0	52.7	58.2	230 bp	1.0	42.8
ycf1_p5	TCGTTTTTAGTTTCACTCCA	TCACCCCAAAACCAACCTTC	35.0	50.0	52.7	58.2	233 bp	1.0	42.7