Hotspot

ndhF

SSC · 108563-110770 · 2208 bp

Back to Sempervivum

Pi 0.00731

MarkerSeek score 42.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2208
variable_sites: 39
pi: 0.00731
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGAGGGATCCACCGAAGAA	AAGGGTTTCCGCTTTTAGCT	50.0	45.0	58.0	57.7	2808-2814 bp	0.666667	64.2
ndhF_p2	CTTCCGAAACGAAGTGGACT	AAGGGTTTCCGCTTTTAGCT	50.0	45.0	57.8	57.7	2838-2844 bp	0.666667	63.4
ndhF_p3	AGGGATCCACCGAAGAAGAT	AAGGGTTTCCGCTTTTAGCT	50.0	45.0	57.8	57.7	2805-2811 bp	0.666667	63.2
ndhF_p4	GGAAGAGATCCGAGTGAACG	AAGGGTTTCCGCTTTTAGCT	55.0	45.0	57.8	57.7	2729-2735 bp	0.666667	63.1
ndhF_p5	AGGGAGGAAAGCAGAGAAGA	AAGGGTTTCCGCTTTTAGCT	50.0	45.0	57.7	57.7	2881-2887 bp	0.666667	61.9