Hotspot

psaC-ndhE

SSC · 115247-115522 · 276 bp

Back to Sempervivum

Pi 0.00679

MarkerSeek score 54.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 276
variable_sites: 5
pi: 0.00679
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CAGGCTCGAACACATTGAGT	CTGAAGCAGCTATTGGACCA	50.0	50.0	58.2	57.6	427-431 bp	1.0	76.6
psaC-ndhE_p2	CAGGCTCGAACACATTGAGT	TGAAGCAGCTATTGGACCAG	50.0	50.0	58.2	57.6	426-430 bp	1.0	76.6
psaC-ndhE_p3	CAGGCTCGAACACATTGAGT	GCAGCTATTGGACCAGCAAT	50.0	50.0	58.2	58.6	422-426 bp	1.0	75.1
psaC-ndhE_p4	TTTAGCTTTACACCCGTCCC	CTGAAGCAGCTATTGGACCA	50.0	50.0	57.5	57.6	476-480 bp	1.0	74.3
psaC-ndhE_p5	TTTAGCTTTACACCCGTCCC	TGAAGCAGCTATTGGACCAG	50.0	50.0	57.5	57.6	475-479 bp	1.0	74.3