Hotspot

trnP-UGG-psaJ

LSC · 65366-65715 · 350 bp

Back to Sempervivum

Pi 0.0134
MarkerSeek score 58.90
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
350
variable_sites
10
pi
0.0134
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG GTCAGCGCATCTGGGAATAA 45.0 50.0 57.9 58.0 464-501 bp 1.0 84.4
trnP-UGG-psaJ_p2 AAAACAAACGCGCTACCAAG TACTTAGTACCGGTGCCACA 45.0 50.0 57.9 58.1 397-434 bp 1.0 84.4
trnP-UGG-psaJ_p3 CAAAACAAACGCGCTACCAA GTCAGCGCATCTGGGAATAA 45.0 50.0 58.2 58.0 465-502 bp 1.0 83.9
trnP-UGG-psaJ_p4 CAAAACAAACGCGCTACCAA TACTTAGTACCGGTGCCACA 45.0 50.0 58.2 58.1 398-435 bp 1.0 83.9
trnP-UGG-psaJ_p5 TTGTACCCAAAACAAACGCG GTCAGCGCATCTGGGAATAA 45.0 50.0 57.8 58.0 472-509 bp 1.0 83.7