Hotspot

psbE-petL

LSC · 63580-64604 · 1025 bp

Back to Sempervivum

Pi 0.0116

MarkerSeek score 55.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 26
pi: 0.0116
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.9	58.0	1215-1219 bp	1.0	81.1
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1138-1142 bp	1.0	80.4
psbE-petL_p3	ACGAATAACCAACCCGCAAT	CGTATCTTGCTCAGCCCAAT	45.0	50.0	58.2	58.0	1224-1228 bp	1.0	80.1
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	CGTATCTTGCTCAGCCCAAT	55.0	50.0	58.2	58.0	1133-1137 bp	1.0	80.1
psbE-petL_p5	CGAATAACCAACCCGCAATG	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1223-1227 bp	1.0	80.0