Hotspot

psbC-trnS-UGA

LSC · 35475-35657 · 183 bp

Back to Sempervivum

Pi 0.0281
MarkerSeek score 60.90
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
183
variable_sites
10
pi
0.0281
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 304-313 bp 1.0 84.6
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.2 58.2 227-236 bp 1.0 84.4
psbC-trnS-UGA_p3 AGCCTGTTCTTTCCATGACC GTCTTTCGGAGAGATGGCTG 50.0 55.0 57.8 58.1 306-315 bp 1.0 84.2
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC TCTTTCGGAGAGATGGCTGA 55.0 50.0 58.2 58.1 303-312 bp 1.0 84.0
psbC-trnS-UGA_p5 AGCCTGTTCTTTCCATGACC TCTTTCGGAGAGATGGCTGA 50.0 50.0 57.8 58.1 305-314 bp 1.0 83.6