Hotspot

trnS-GCU-trnG-GCC

LSC · 8758-9396 · 639 bp

Back to Sempervivum

Pi 0.0166
MarkerSeek score 63.70
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
639
variable_sites
23
pi
0.0166
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 620-696 bp 1.0 54.7
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 619-695 bp 1.0 54.7
trnS-GCU-trnG-GCC_p3 GCTCGTACAACGGATTAGCA TACCACTAAACTATACCCGC 50.0 45.0 58.1 54.0 638-714 bp 1.0 54.0
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 634-710 bp 1.0 53.8
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 633-709 bp 1.0 53.8