Hotspot

rps16-trnQ-UUG

LSC · 5876-7411 · 1536 bp

Back to Sempervivum

Pi 0.0138
MarkerSeek score 63.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1536
variable_sites
50
pi
0.0138
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1149-1617 bp 1.0 84.9
rps16-trnQ-UUG_p2 CAACTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 1156-1624 bp 0.833333 75.9
rps16-trnQ-UUG_p3 CTTCAACTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1159-1627 bp 0.833333 75.8
rps16-trnQ-UUG_p4 TCAACTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 1157-1625 bp 0.833333 75.3
rps16-trnQ-UUG_p5 TTCAACTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 1158-1626 bp 0.833333 75.3