Hotspot

trnK-UUU-rps16

LSC · 4122-4801 · 680 bp

Back to Sempervivum

Pi 0.0167
MarkerSeek score 64.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
680
variable_sites
27
pi
0.0167
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAGGCGGATGTTTTTAGGGA 50.0 45.0 58.2 57.1 745-771 bp 1.0 77.4
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AGGCGGATGTTTTTAGGGAA 50.0 45.0 58.2 57.1 744-770 bp 1.0 77.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAGGCGGATGTTTTTAGGGA 50.0 45.0 58.2 57.1 744-770 bp 1.0 77.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGGCGGATGTTTTTAGGGAA 50.0 45.0 58.2 57.1 743-769 bp 1.0 77.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGCGGATGTTTTTAGGGAAC 50.0 50.0 58.2 57.1 742-768 bp 1.0 77.1