Hotspot

trnH-GUG-psbA

LSC · 73-311 · 239 bp

Back to Sempervivum

Pi 0.0477
MarkerSeek score 74.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
239
variable_sites
23
pi
0.0477
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG ATTTCTTCCTAGCTGCGTGG 55.0 50.0 58.1 58.0 426-525 bp 1.0 86.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA ATTTCTTCCTAGCTGCGTGG 50.0 50.0 57.9 58.0 440-539 bp 1.0 86.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC ATTTCTTCCTAGCTGCGTGG 50.0 50.0 57.9 58.0 439-538 bp 1.0 86.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC ATTTCTTCCTAGCTGCGTGG 50.0 50.0 57.9 58.0 441-540 bp 1.0 86.5
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CGTGGCCTGTAGTTGGTATC 55.0 55.0 58.1 58.1 411-510 bp 1.0 86.5