Hotspot

rps8

LSC · 84798-85202 · 405 bp

Back to Semiliquidambar

Pi 0

MarkerSeek score 22.20

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	GAATCCTTGTTGCGGAGTCT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.9	58.0	1015-1017 bp	1.0	79.4
rps8_p2	TACGTCGGATCCTTCCTGAA	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.9	58.0	915-917 bp	1.0	79.1
rps8_p3	AACTACGTCGGATCCTTCCT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.9	58.0	918-920 bp	1.0	79.1
rps8_p4	TCGGGTCCTTTAGAGAGTCC	GAACTCGAGTTTTTGGTGCG	55.0	50.0	57.9	58.0	1305-1307 bp	1.0	79.0
rps8_p5	CGGGTCCTTTAGAGAGTCCT	GAACTCGAGTTTTTGGTGCG	55.0	50.0	57.9	58.0	1304-1306 bp	1.0	79.0