Hotspot

rpoA

LSC · 82577-83590 · 1014 bp

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Pi 0.00197

MarkerSeek score 59.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 2
pi: 0.00197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CGCTTCAAAGTGAATTCTCCC	CCACATAATGGCTGTAGACCT	47.6	47.6	57.5	57.2	1219 bp	1.0	53.5
rpoA_p2	CGCTTCAAAGTGAATTCTCCC	ACATAATGGCTGTAGACCTCC	47.6	47.6	57.5	56.9	1217 bp	1.0	51.3
rpoA_p3	CGCTTCAAAGTGAATTCTCCC	CACATAATGGCTGTAGACCTCC	47.6	50.0	57.5	58.3	1218 bp	1.0	50.2
rpoA_p4	CGCTTCAAAGTGAATTCTCCC	CCACATAATGGCTGTAGACCTC	47.6	50.0	57.5	58.3	1219 bp	1.0	50.2
rpoA_p5	CGCTTCAAAGTGAATTCTCCC	ACATAATGGCTGTAGACCTCCT	47.6	45.5	57.5	58.3	1217 bp	1.0	49.6