Hotspot

ndhI-ndhA

SSC · 125391-125468 · 78 bp

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Pi 0.0128

MarkerSeek score 59.90

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 78
variable_sites: 1
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGCAGCTCGTACTGTTTGTT	ACCTAGGCTGAGAATGGACC	45.0	55.0	58.1	58.5	244 bp	1.0	75.9
ndhI-ndhA_p2	TTGCAGCTCGTACTGTTTGT	ACCTAGGCTGAGAATGGACC	45.0	55.0	58.1	58.5	245 bp	1.0	75.9
ndhI-ndhA_p3	TGCAGCTCGTACTGTTTGTT	CCTAGGCTGAGAATGGACCA	45.0	55.0	58.1	58.5	243 bp	1.0	75.9
ndhI-ndhA_p4	TTGCAGCTCGTACTGTTTGT	CCTAGGCTGAGAATGGACCA	45.0	55.0	58.1	58.5	244 bp	1.0	75.9
ndhI-ndhA_p5	ACCAATGTACCTTGCAGCTC	ACCTAGGCTGAGAATGGACC	50.0	55.0	58.2	58.5	256 bp	1.0	74.9