Hotspot

ndhF-rpl32

SSC · 117494-118562 · 1069 bp

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Pi 0.00281
MarkerSeek score 60.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1069
variable_sites
3
pi
0.00281
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTGTCGGAAAAAGGA AAAAGCTTTTAACGCTGCCC 45.0 45.0 58.0 57.9 1263-1264 bp 1.0 78.7
ndhF-rpl32_p2 TGTTGCTGTCGGAAAAAGGA AAGCTTTTAACGCTGCCCTA 45.0 45.0 58.0 57.8 1261-1262 bp 1.0 78.3
ndhF-rpl32_p3 TGTTGCTGTCGGAAAAAGGA TAACGCTGCCCTATATCCCT 45.0 50.0 58.0 57.7 1254-1255 bp 1.0 77.3
ndhF-rpl32_p4 TGTTGCTGTCGGAAAAAGGA GCTGCCCTATATCCCTTCCT 45.0 55.0 58.0 58.3 1250-1251 bp 1.0 77.2
ndhF-rpl32_p5 CTGGAAGTGGAACGAAAGGT AAAAGCTTTTAACGCTGCCC 50.0 45.0 57.7 57.9 1216-1217 bp 1.0 77.0