Hotspot

rps8-rpl14

LSC · 85203-85383 · 181 bp

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Pi 0.0112

MarkerSeek score 60.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 2
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.7	58.0	286-288 bp	1.0	78.9
rps8-rpl14_p2	AGCAATAGTGTCCCTACCCA	TCATTAGCCCCCGAGGTATT	50.0	50.0	57.7	57.9	224-226 bp	1.0	78.4
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	TTCATTAGCCCCCGAGGTAT	50.0	50.0	57.7	57.9	225-227 bp	1.0	78.4
rps8-rpl14_p4	AGCAATAGTGTCCCTACCCA	TTTCATTAGCCCCCGAGGTA	50.0	50.0	57.7	58.1	226-228 bp	1.0	78.4
rps8-rpl14_p5	AGCAATAGTGTCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	57.7	58.1	284-286 bp	1.0	78.0