Hotspot

rpl20-rps12

LSC · 73616-74405 · 790 bp

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Pi 0.00127
MarkerSeek score 59.70
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
790
variable_sites
1
pi
0.00127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTTGTTCTACGTCTCCGAGC CGCCGAGGAACATGTACTAG 50.0 55.0 57.7 57.9 862-864 bp 1.0 76.6
rpl20-rps12_p2 TGTTCTACGTCTCCGAGCTA CGCCGAGGAACATGTACTAG 50.0 55.0 57.6 57.9 860-862 bp 1.0 76.2
rpl20-rps12_p3 TTGTTCTACGTCTCCGAGCT CGCCGAGGAACATGTACTAG 50.0 55.0 58.5 57.9 861-863 bp 1.0 75.6
rpl20-rps12_p4 CCCGAAAGCTTGATGCAAAT CGCCGAGGAACATGTACTAG 45.0 55.0 57.3 57.9 891-893 bp 1.0 74.0
rpl20-rps12_p5 TTTGTTCTACGTCTCCGAGC GCCGAGGAACATGTACTAGG 50.0 55.0 57.7 57.5 861-863 bp 1.0 73.4