Hotspot

tRNA-UGG-psaJ

LSC · 71281-71710 · 430 bp

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Pi 0
MarkerSeek score 59.70
Primer available yes
Label tRNA-UGG-psaJ

Feature Metadata

label_name
tRNA-UGG-psaJ
feature_type
igs
region
LSC
length_bp
430
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GACCTGCTAAAGACCCGAAC 50.0 55.0 58.1 58.3 538-558 bp 1.0 79.9
tRNA-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GACCTGCTAAAGACCCGAAC 50.0 55.0 58.1 58.3 537-557 bp 1.0 79.9
tRNA-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GACCTGCTAAAGACCCGAAC 50.0 55.0 58.1 58.3 549-569 bp 1.0 79.8
tRNA-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GACCTGCTAAAGACCCGAAC 50.0 55.0 58.1 58.3 548-568 bp 1.0 79.8
tRNA-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GACCTGCTAAAGACCCGAAC 50.0 55.0 58.1 58.3 551-571 bp 1.0 79.8