Hotspot

accD-psaI

LSC · 63032-63475 · 444 bp

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Pi 0.00677

MarkerSeek score 59.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 444
variable_sites: 3
pi: 0.00677
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	ACTAAAGGCACCAAGACAGC	50.0	50.0	58.4	58.1	552-553 bp	1.0	76.8
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.4	57.9	588-589 bp	1.0	76.7
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	TTGCCATTGCCGGAAATACT	50.0	45.0	58.4	58.2	578-579 bp	1.0	76.4
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	CAAGACAGCGGGTAAGTTGA	50.0	50.0	58.4	57.8	541-542 bp	1.0	76.3
accD-psaI_p5	AGGGCGTTCTGAGTGAGTTA	CCACTAAAGGCACCAAGACA	50.0	50.0	58.4	57.7	554-555 bp	1.0	75.4