Hotspot

tRNA-GAA-ndhJ

LSC · 52026-52758 · 733 bp

Back to Semiliquidambar

Pi 0.00136

MarkerSeek score 59.50

Primer available yes

Label tRNA-GAA-ndhJ

Feature Metadata

label_name: tRNA-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	907 bp	1.0	79.6
tRNA-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	871 bp	1.0	79.2
tRNA-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	895 bp	1.0	78.5
tRNA-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GAAAGTTGGATAGGGTGGCC	55.0	55.0	58.0	58.2	878 bp	1.0	78.2
tRNA-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	CCACGCCTGAAACGTATCTT	55.0	50.0	58.0	58.3	905 bp	1.0	77.8