Hotspot

ndhF

SSC · 117085-119304 · 2220 bp

Back to Semiaquilegia

Pi 0.0104

MarkerSeek score 48.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 23
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	ATAAGAGCGGGTGAATCGGA	50.0	50.0	58.0	58.6	2564-2569 bp	1.0	76.2
ndhF_p2	ACAGGAACAAGAGGGATCCA	ATAAGAGCGGGTGAATCGGA	50.0	50.0	58.0	58.6	2550-2555 bp	1.0	76.0
ndhF_p3	AAGAGGGATCCACCGAAGAA	ATAAGAGCGGGTGAATCGGA	50.0	50.0	58.0	58.6	2542-2547 bp	1.0	75.9
ndhF_p4	CCTTCCCCTTCCCTTTGTTC	ATAAGAGCGGGTGAATCGGA	55.0	50.0	58.1	58.6	2519-2524 bp	1.0	75.7
ndhF_p5	AGTTCAACGTTCGCCAGATT	ATAAGAGCGGGTGAATCGGA	45.0	50.0	58.1	58.6	2957-2962 bp	1.0	75.3