Hotspot

ccsA-ndhD

SSC · 121824-122052 · 229 bp

Back to Semiaquilegia

Pi 0.0263

MarkerSeek score 68.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 229
variable_sites: 6
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGGGCTGCATAGTTATGGTT	TCCGGATTTCGTTCTCTCAC	50.0	50.0	58.0	57.4	351-355 bp	1.0	76.1
ccsA-ndhD_p2	CGGGCTGCATAGTTATGGTT	CCGGATTTCGTTCTCTCACT	50.0	50.0	58.0	57.3	350-354 bp	1.0	76.1
ccsA-ndhD_p3	CTGCAATTGTGGCTTCGATG	TCCGGATTTCGTTCTCTCAC	50.0	50.0	58.1	57.4	421-425 bp	1.0	75.4
ccsA-ndhD_p4	CTGCAATTGTGGCTTCGATG	CCGGATTTCGTTCTCTCACT	50.0	50.0	58.1	57.3	420-424 bp	1.0	75.3
ccsA-ndhD_p5	TCTGCAATTGTGGCTTCGAT	TCCGGATTTCGTTCTCTCAC	45.0	50.0	58.2	57.4	422-426 bp	1.0	74.7