Hotspot

ndhF-trnL-UAG

SSC · 119305-120651 · 1347 bp

Back to Semiaquilegia

Pi 0.023

MarkerSeek score 65.00

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1347
variable_sites: 29
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	ATAGGAACTGGAAGGGGGAC	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	1402-1435 bp	1.0	84.7
ndhF-trnL-UAG_p2	GGAGAAGACCCACCCCTATT	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	1425-1458 bp	1.0	84.7
ndhF-trnL-UAG_p3	ATAGGAACTGGAAGGGGGAC	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	1403-1436 bp	1.0	84.7
ndhF-trnL-UAG_p4	GGAGAAGACCCACCCCTATT	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	1426-1459 bp	1.0	84.7
ndhF-trnL-UAG_p5	GAACAAGGAGAAGACCCACC	AGCGTGTCTACCGATTTCAC	55.0	50.0	57.8	58.0	1431-1464 bp	1.0	84.0