Hotspot

rps8-rpl14

LSC · 86443-86671 · 229 bp

Back to Semiaquilegia

Pi 0.0369

MarkerSeek score 64.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 8
pi: 0.0369
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TGTCGCAGAGTTGAAACCAA	AATCAAAGAAGCAGTGCCCA	45.0	45.0	58.0	58.0	661-667 bp	1.0	82.4
rps8-rpl14_p2	TGTCGCAGAGTTGAAACCAA	ATCAAAGAAGCAGTGCCCAA	45.0	45.0	58.0	58.0	660-666 bp	1.0	82.4
rps8-rpl14_p3	TGTCGCAGAGTTGAAACCAA	TCAAAGAAGCAGTGCCCAAT	45.0	45.0	58.0	58.0	659-665 bp	1.0	82.4
rps8-rpl14_p4	TGTCGCAGAGTTGAAACCAA	GTAATCGCCGATATGCTCGT	45.0	50.0	58.0	58.0	704-710 bp	1.0	82.1
rps8-rpl14_p5	TGTCGCAGAGTTGAAACCAA	TCGCCGATATGCTCGTATTG	45.0	50.0	58.0	58.0	700-706 bp	1.0	82.1