Hotspot

psaJ-rpl33

LSC · 70842-71274 · 433 bp

Back to Semiaquilegia

Pi 0.0164

MarkerSeek score 66.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 7
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	AATGCCCCGCGATTCTTTAT	45.0	45.0	58.1	58.0	556-561 bp	1.0	81.1
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	ATGCCCCGCGATTCTTTATT	45.0	45.0	58.1	58.0	555-560 bp	1.0	81.1
psaJ-rpl33_p3	CTCTATGGTTCGGGTCGTTG	AATGCCCCGCGATTCTTTAT	55.0	45.0	58.1	58.0	613-618 bp	1.0	80.9
psaJ-rpl33_p4	CTCTATGGTTCGGGTCGTTG	ATGCCCCGCGATTCTTTATT	55.0	45.0	58.1	58.0	612-617 bp	1.0	80.9
psaJ-rpl33_p5	TATCTATCCGTGGCACCTGT	ATGCCCCGCGATTCTTTATT	50.0	45.0	57.9	58.0	640-645 bp	1.0	80.8