Hotspot

trnP-UGG-psaJ

LSC · 70292-70706 · 415 bp

Back to Semiaquilegia

Pi 0.0196
MarkerSeek score 67.80
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
415
variable_sites
8
pi
0.0196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC ACAGGTGCCACGGATAGATA 50.0 50.0 58.1 57.9 506-508 bp 1.0 80.9
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA ACAGGTGCCACGGATAGATA 50.0 50.0 58.1 57.9 505-507 bp 1.0 80.9
trnP-UGG-psaJ_p3 ACCCGTGACATTTTGTACCC CAACGACCCGAACCATAGAG 50.0 55.0 58.1 58.1 534-536 bp 1.0 80.8
trnP-UGG-psaJ_p4 CCCGTGACATTTTGTACCCA CAACGACCCGAACCATAGAG 50.0 55.0 58.1 58.1 533-535 bp 1.0 80.8
trnP-UGG-psaJ_p5 ACAGGATTTGAACCCGTGAC CAACGACCCGAACCATAGAG 50.0 55.0 58.1 58.1 545-547 bp 1.0 80.7