Hotspot

psbE-petL

LSC · 68302-69370 · 1069 bp

Back to Semiaquilegia

Pi 0.00947

MarkerSeek score 65.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 10
pi: 0.00947
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACGAATAACCAACCCGCAAT	GCATGAAGGAGCTAAGTGGG	45.0	55.0	58.2	58.3	1209-1222 bp	1.0	77.5
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GGCATGAAGGAGCTAAGTGG	45.0	55.0	58.2	58.3	1210-1223 bp	1.0	77.5
psbE-petL_p3	TGCTGACGAATAACCAACCC	GCATGAAGGAGCTAAGTGGG	50.0	55.0	58.2	58.3	1214-1227 bp	1.0	77.4
psbE-petL_p4	TGCTGACGAATAACCAACCC	GGCATGAAGGAGCTAAGTGG	50.0	55.0	58.2	58.3	1215-1228 bp	1.0	77.4
psbE-petL_p5	CGAATAACCAACCCGCAATG	GCATGAAGGAGCTAAGTGGG	50.0	55.0	57.8	58.3	1208-1221 bp	1.0	77.4