Hotspot

rbcL-accD

LSC · 57780-61194 · 3415 bp

Back to Semiaquilegia

Pi 0.0115

MarkerSeek score 66.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 3415
variable_sites: 37
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCCAAGCAAGCTCTACCCTA	GGCACACCTTCTCAATCGAA	55.0	50.0	58.8	58.2	242-253 bp	1.0	78.6
rbcL-accD_p2	CCAAGCAAGCTCTACCCTAT	GGCACACCTTCTCAATCGAA	50.0	50.0	56.7	58.2	241-252 bp	1.0	74.5
rbcL-accD_p3	CCCAAGCAAGCTCTACCCTA	GCACACCTTCTCAATCGAAC	55.0	50.0	58.8	57.1	241-252 bp	1.0	73.1
rbcL-accD_p4	CCCAAGCAAGCTCTACCCTA	AGTCGAATCCTAGATGGGGA	55.0	50.0	58.8	56.9	276-287 bp	1.0	70.7
rbcL-accD_p5	CCCAAGCAAGCTCTACCCTA	TGGCACACCTTCTCAATCG	55.0	52.6	58.8	57.8	243-254 bp	1.0	70.2