Hotspot

atpF-atpH

LSC · 12714-13182 · 469 bp

Back to Semiaquilegia

Pi 0.0245

MarkerSeek score 74.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 11
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.0	58.0	548-564 bp	1.0	83.8
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	576-592 bp	1.0	83.0
atpF-atpH_p3	AGTGACCCAAGGAAACGAAA	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.3	58.0	547-563 bp	1.0	78.4
atpF-atpH_p4	TGACCCAAGGAAACGAAAGA	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.0	58.0	545-561 bp	1.0	76.1
atpF-atpH_p5	GTGACCCAAGGAAACGAAAG	ATTTATGGACTGGTCGTGGC	50.0	50.0	56.9	58.0	546-562 bp	1.0	75.7