Hotspot

ndhF

Genome · 113034-115271 · 2238 bp

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Pi 0.00411

MarkerSeek score 46.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2238
variable_sites: 23
pi: 0.00411
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTGAGGCTCTAGATACGGGA	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.0	58.3	2878-2939 bp	1.0	72.8
ndhF_p2	AATTGGAAGGGCCTCTCTTT	GGGGGTAGGATCCGGTAAAT	45.0	55.0	56.7	58.3	2522-2583 bp	1.0	71.1
ndhF_p3	AAATTGGAAGGGCCTCTCTT	GGGGGTAGGATCCGGTAAAT	45.0	55.0	56.7	58.3	2523-2584 bp	1.0	71.0
ndhF_p4	TGAGGCTCTAGATACGGGAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	56.7	58.3	2877-2938 bp	1.0	70.9
ndhF_p5	CGAGTTTAAATTGGAAGGGCC	GGGGGTAGGATCCGGTAAAT	47.6	55.0	57.5	58.3	2530-2591 bp	1.0	69.5