Hotspot

trnV-rrn16

Genome · 100637-102448 · 1812 bp

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Pi 0.0148

MarkerSeek score 55.10

Primer available yes

Label trnV-rrn16

Feature Metadata

label_name: trnV-rrn16
feature_type: igs
region: Genome
length_bp: 1812
variable_sites: 40
pi: 0.0148
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-rrn16_p1	TGAGTTTCTCGACCCTTTGC	CCGACTTGCATGTGTTAAGC	50.0	50.0	58.1	57.8	413-2071 bp	1.0	83.2
trnV-rrn16_p2	TTGACGTGGTGGAAGTCATC	CCGACTTGCATGTGTTAAGC	50.0	50.0	57.8	57.8	323-1981 bp	1.0	83.0
trnV-rrn16_p3	CGTGGTGGAAGTCATCAGTT	CCGACTTGCATGTGTTAAGC	50.0	50.0	57.8	57.8	319-1977 bp	1.0	83.0
trnV-rrn16_p4	CTTGACGTGGTGGAAGTCAT	CCGACTTGCATGTGTTAAGC	50.0	50.0	57.8	57.8	324-1982 bp	1.0	83.0
trnV-rrn16_p5	TGAGTTTCTCGACCCTTTGC	GCAGGTTCTTACGCGTTACT	50.0	50.0	58.1	58.3	466-2124 bp	1.0	82.8